Methylgyoxal (MG) is reactive dicarbonyl compound was found in high level in blood of diabetic patients. Methlyglyoxal was more reactive compared to glucose and have toxic effect through AGEs. Soluble Receptor for Advanced Glycation End Products (sRAGE) act as a scavenger and decoy receptor for AGEs. This study aimed to investigate the effect of changes intracellularly redox status on sRAGE levels in preosteoblast MC3T3E1 exposed to MG. The changes of redox status was obtained by blockade of superoxide dismutase (Diethylthiocarbamoic acid/DETCA), blockade of gluthatione peroxidase (mercapto succinate/MS), and chelating of iron (defferoxamine/DFX). sRAGE levels was evaluated by enzyme linked imunnosorbent assay technique. The levels of sRAGE in MG-exposed group or DETCA-exposed group was significantly increased than that in control group (P < 0.05). The levels of sRAGE in MG+DETCA-exposed group was significantly decrease compared with DETCA-exposed group (P < 0.05). The levels of sRAGE was significantly increased in MS-exposed group or MG+MS-exposed group than that in control group, respectively (P < 0.05). Concluded that the change of redox status by antioxidant blocker or MG exposure would increases sRAGE levels in preosteoblast MC3T3E1 cell line. The modulation of redox state by antioxidant blocker does not affect sRAGE level in the presence of MG. Iron chelating does not change the sRAGE level.
Keywords: preosteoblast MC3T3E1 cell line; methylgyoxal; reactive oxygen species; sRAGE
