Original Article

Chitin increases the angiogenesis in chorioallantoic membrane model in the presence of testosterone and progesterone


  • Ezgi İrem Sağlam
  • İlayda Ceren Kutlu
  • Orhan Erdem Haberal
  • Mehmet Yüksekkaya
  • Özgür Kılıçarslan
  • Şefik Güran

Received Date: 17.08.2018 Accepted Date: 28.09.2018 Gulhane Med J 2019;61(1):20-24


Angiogenesis is a process of generating new blood vessels from preexisting vessels. In adults, it is activated in pathologic conditions. Chitin is an organic molecule which is used in scaffold technology in tissue engineering. Growth hormones such as testosterone and progesterone are used in scaffold structure for induction of angiogenesis. No literature was found about the angiogenic roles of chitin/testosterone/ progesterone. Here, chitin was analyzed in the presence of testosterone/progesterone to find out its’ possible role on angiogenesis.


Chitin was obtained from shrimp shells in our laboratory. The angiogenic effects of chitin/ testosterone/progesterone were analyzed on chick embryo chorioallontoic membrane (CAM) model. Six different study groups were prepared (control group-Group 1, testosterone applied group-Group 2, progesterone applied group-Group 3, chitin/testosterone applied group-Group 4, chitin/progesterone applied group-Group 5, chitin/testosterone/progesterone applied group-Group 6). Hormones were used in different concentrations. The angiogenic role of selected molecules was clarified according to the total differentiation score of angiogenesis (TDSA) results in all groups. In obtaining of TDSA results, Knighton’s protocol was applied.


TDSA was 6±0.1 in testosterone applied group, 5±0.2 in progesterone applied group, 7±0.1 in chitin/testosterone applied group, 5±0.1 in chitin/progesterone applied group, 7±0.1 in chitin/ testosterone/progesterone applied group. In all groups, TDSA results were statistically significant. These results represented the angiogenic role of chitin in the presence of testosterone and progesterone (p<0.05).


Our results support the angiogenic roles of chitin in the presence of testosterone and progesterone. Chitin, testosterone and progesterone can be used in scaffold technology together.

Keywords: Chitin, testosterone, progesterone, chorioallantoic membrane, angiogenesis, tissue engineering.