From July 2000 to Dec. 2001, a total of 21600 urine specimens were collected from inpatients and outpatients of King Hussein Medical Center, located in Amman/Jordan for culture and antimicrobial susceptibility testing. Among these, 2537 specimens (11.74%) showed significant bacteriuria. The ratio of female-to- male patients was 1523:1014 (1.50:1.00). The most frequently recovered pathogens were E.coli (58.90%), Klebsiella pneumoniae (12.90%), Pseudomonus aeruginosa (5.20%), Enterococcus fecalis (4.80%) and Proteus mirabilis (4.20%) . In this study, all isolates were tested for their antimicrobial susceptibilities against different groups of antibiotics, with Vitek-1 system (Bio Merieux, France) to determine their susceptibility patterns. As a result, (88.00%) of the Gram-negative isolates other than Pseudomonas aeruginosa were found to be respectively resistant to carbenicillin, (87.00%) to ampicillin, (78.00%) to cephalothin, (75.00%) to cefazolin and (60.00%) to trimethoprim/sulphamethoxazole.

Keywords: Vitek, Uropathojen, Susceptibility