Original Article

The sitotoxic effect of gemcitabine on multiple myeloma (RPMI-8226) and Ig G plasma cell leukemia (ARH 77) cell lines

10.5455/gulhane.12923

  • Zehra Dilşad Çoban
  • Ferit Avcu
  • Ali Uğur Ural
  • Okan Kuzhan
  • Şefik Güran

Received Date: 01.02.2012 Accepted Date: 23.03.2012 Gulhane Med J 2012;54(4):263-266

Gemcitabine replaces one of the nucleic acids such as cytidine as a nucleoside analog during DNA replication. The process arrests tumor growth resulting in apoptosis. As a chemotherapeutic agent, gemcitabine has systemic effect on every tumor cells. So, it is used in various carcinomas: non-small cell lung cancer, pancreatic cancer, bladder cancer and breast cancer. Here, multiple myeloma-RPMI-8226 and Ig G plasma cell leukemia- ARH 77 cell lines were treated with gemcitabine in different concentrations. Cytotoxic effect of gemcitabine was detected by XTT assay in cell culture. In RPMI-8226 cell line, lethal effect was detected at the concentration of 400 μmol/L. In ARH-77 cell line, lethal effect was found at the concentration of 200 μmol/L. These results represent us the cytotoxic affect of gemcitabine on multiple myeloma and Ig G plasma cell leukemia tumor cells. It is needed to do further experiments for finding efficiency of multiple myeloma and plasma cell leukemia treatment with gemcitabine.

Keywords: Gemcitabine, RPMI-8226, ARH 77, nucleoside analog, multiple myeloma, Ig G plasma cell leukemia