Objective:
Predominantly circulation problems as well as a number of reasons may cause chronic wounds by impairing wound healing process. In this study, we aimed to investigate the effects of two similar wound dressing materials with and without honey and their combination with hyperbaric oxygen (HBO) therapy on chronic wound healing.
Material and Methods:
For this purpose, chronic wound model was established on 42 Sprague-Dawley male rats and divided into six groups as follows: Control group without any treatment, HBO treatment group, wound dressing (Dermax®) group, wound dressing with honey (Melmax®) group,Dermax® plus HBO treatment group and Melmax® plus HBO group. HBO treatment was administered two times a day under 2.5 ATA, for 60 minutes. Wound dressing change was performed once in every day. At the eighth day of applications, wound tissues were excised for evaluation under anesthesia.
Results:
Evaluation of wound surface area, biochemical (malondialdehyde and superoxide dismutase) and histopathological (inflammation, epithelization, collagen synthesis, angiogenesis) parameters showed an apparent healing at all treatment groups when compared with control group. Groups that received combination treatment with wound dressings and HBO were found to have increased wound healing and the difference was statistically significant.
Conclusion:
In our study, we found that wound dressing applications accelerated the wound healing when compared to control group and HBO treatment combination with wound dressings strengthened the healing process.
Keywords: Wound Healing; Oxidative Stress; PHI-5 formulation
